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The enzyme cleaves 1-4 linkages between N-acetylated or N-sulphated glucosamine and glucuronic acid residues. It is only active towards heparan sulfate in the low sulphated zone and does not cleave heparin or low molecular weight heparins. Highly sulfated zones are resistant to it and activity towards iduronic acid is very low.Stabilised with 0.2-0.4% BSA (Sigma A4378), 0.22 µm sterile filtered and dispensed into sterile tubes/vials.