CarboClip is a 37.2 kDa recombinant peptide-N-Glycosidase F (PNGase F), from Elizabethkingia sp., overexpressed in E. coli that cleaves between the GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.
CarboClip is provided glycerol-free as a lyophilized powder containing Tris-HCl, NaCl2, and Na2EDTA.
Activity: After reconstitution in 150 μL of UltraPure H2O, 1 μl of CarboClip can deglycosylate > 95% of ribonuclease B (1 mg) in less than 20 minutes. Activity is calculated on a 12% SDS-PAGE and percent of deglycosylation using densitometry.
For additional information, please download our information sheet.
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