A range of N-glycans labelled with 13C, for use as quantitative standards in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS). These are available as the single standards or in kit form.
Advantages of CarboQuant standards
- Label-free glycan quantification by MS
- Absolute quantification of individual glycans in complex mixtures (e.g. glycan biomarkers)
- No external calibration required, use as internal standard due to stable isotope enrichment
- Standards are quantified by qNMR
- Custom-made software for rapid automated quantification available
- Stable isotope enrichment in glycan core and antennae for fragment quantification
- Degree of isotopic enrichment can be tuned during synthesis
- Method transfer between labs and technicians
- 4-8 Da mass increment of heavy isoptopologue avoids signal overlap between analyte and standard MS signals
- Standards provided as single isomers, no isobaric mixtures
- Fully synthetic and characterized by NMR, HPLC and MS, high purity (>95%)
- Custom synthesis of standards offered
- Compatible with labelling by reductive amination, permethylation, sialic acid derivatisation
Applications
- Measure serum glycan levels as disease markers by MS
- Quantify absolute and relative glycosylation in biopharmaceutical development and quality control
- Glycan biomarker discovery by MALDI (cross-quantification)
- Increase reproducibility in Lab-to-lab method transfer (internal calibration standard)
- De-convolute and quantify co-eluting peaks in LC-MS
- Quantify glycan recovery after sample preparation
- Protein characterization
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