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13C Labelled Glycans

A range of N-glycans labelled with 13C, for use as quantitative standards in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS). These are available as the single standards or in kit form.

Advantages of CarboQuant standards

  • Label-free glycan quantification by MS
  • Absolute quantification of individual glycans in complex mixtures (e.g. glycan biomarkers)
  • No external calibration required, use as internal standard due to stable isotope enrichment
  • Standards are quantified by qNMR
  • Custom-made software for rapid automated quantification available
  • Stable isotope enrichment in glycan core and antennae for fragment quantification
  • Degree of isotopic enrichment can be tuned during synthesis
  • Method transfer between labs and technicians
  • 4-8 Da mass increment of heavy isoptopologue avoids signal overlap between analyte and standard MS signals
  • Standards provided as single isomers, no isobaric mixtures
  • Fully synthetic and characterized by NMR, HPLC and MS, high purity (>95%)
  • Custom synthesis of standards offered
  • Compatible with labelling by reductive amination, permethylation, sialic acid derivatisation


  • Measure serum glycan levels as disease markers by MS
  • Quantify absolute and relative glycosylation in biopharmaceutical development and quality control
  • Glycan biomarker discovery by MALDI (cross-quantification)
  • Increase reproducibility in Lab-to-lab method transfer (internal calibration standard)
  • De-convolute and quantify co-eluting peaks in LC-MS
  • Quantify glycan recovery after sample preparation
  • Protein characterization
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