13C Labelled Glycans

A range of N-glycans labelled with ¹³C, for use as quantitative standards in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS). These are available as the single standards or in kit form.

Advantages of CarboQuant standards

• Label-free glycan quantification by MS
• Absolute quantification of individual glycans in complex mixtures (e.g. glycan biomarkers)
• No external calibration required, use as internal standard due to stable isotope enrichment
• Standards are quantified by qNMR
• Custom-made software for rapid automated quantification available
• Stable isotope enrichment in glycan core and antennae for fragment quantification
• Degree of isotopic enrichment can be tuned during synthesis
• Method transfer between labs and technicians
• 4-8 Da mass increment of heavy isoptopologue avoids signal overlap between analyte and standard MS signals
• Standards provided as single isomers, no isobaric mixtures
• Fully synthetic and characterized by NMR, HPLC and MS, high purity (>95%)
• Custom synthesis of standards offered
• Compatible with labelling by reductive amination, permethylation, sialic acid derivatisation

Applications

• Measure serum glycan levels as disease markers by MS
• Quantify absolute and relative glycosylation in biopharmaceutical development and quality control
• Glycan biomarker discovery by MALDI (cross-quantification)
• Increase reproducibility in Lab-to-lab method transfer (internal calibration standard)
• De-convolute and quantify co-eluting peaks in LC-MS
• Quantify glycan recovery after sample preparation
• Protein characterization