Custom neoglycoprotein service
We are able to link a wide range of carbohydrates to proteins and offer a custom service that can be tailored to meet clients' specific requirements. For example, in the past we have made a considerable variety of neoglycoproteins using complex plant derived oligosaccharides and carbohydrate tumour antigens through to simple monosaccharides. Our neoglycoproteins are frequently used as the solid phase in ELISA assays and we can provide experimental details in this application if required. We can also manufacture our existing range of neoglycoproteins on a larger scale.
Our present methods of choice link carbohydrates via the anomeric position to the ε-amino groups of lysine residues or to sulfydryl groups and we offer a choice of two linker arms. One is 3-atoms and sometimes is the choice for antigen presentation while the other is 14-atoms, frequently preferred as an enzyme acceptor. We aim to link at least eight carbohydrate residues per protein molecule if the target amino acid is lysine. As a general rule, the coupling ratio decreases with increasing molecular weight of carbohydrate due to a combination of poorer reactivity and increasing steric hindrance. If the target group is sulfydryl, then all free SH groups on the protein are usually reacted. We can discuss other coupling options, depending on specific applications. In terms of protein the vast majority of requests are for BSA, HSA or other species’ serum albumins. We can use other proteins depending on their amenability to analysis and the availability of reactive groups. There are some restrictions that apply to the present techniques. The link between carbohydrate and protein is always β. A number of carbohydrates, such as oligosaccharides with a fucose proximal to the anomeric residue will only react poorly. Our present minimum scale will yield 5 to 10 mg of neoglycoprotein. If you would like to discuss your specific requirements, please contact us and we will be happy to provide you with a quotation.
All of our neoglycoproteins are analysed by matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF). This permits the determination of the number of covalently added molecules of antigen per molecule of carrier protein, together with the molecular weight distribution (maximum and minimum numbers of residues per molecule). Generally, neoglycoproteins have an average of 10 to 12 carbohydrates per molecule of protein, with a range of between 7 or 8 to 18 or 20.
List of Neoglycoproteins
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